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docs:manual [2019/02/21 02:36]
Melissa Glidewell improving modularity 		docs:manual [2019/03/11 16:37] (current)
Jon Daniels [Optical alignment]
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 {{section>modular_manual#camera_setup&noheader}} {{section>modular_manual#camera_setup&noheader}}
  
-==== Mount ====+==== Mount Camera Tube Lenses ====
 Tools: M5 hex driver\\  Tools: M5 hex driver\\ 
 In the standard configuration for diSPIM on a RAMM frame, as in <imgref SPIM_Figure>, each camera tube lens is fastened to the SPIM-MOUNT((Other common configurations use a post mount system to minimize vibration from the camera's fans, or to accommodate an FTP system in lieu of a RAMM frame.)) with a ring clamp. The end of the tubes should come within approximately 1 mm from the upper (mirror) CUBE-IIIs. When the objectives are lowered into position for viewing a sample, adjust the bolts holding the tube support arms so the camera tube lenses are centered on the CUBE-IIIs. \\  In the standard configuration for diSPIM on a RAMM frame, as in <imgref SPIM_Figure>, each camera tube lens is fastened to the SPIM-MOUNT((Other common configurations use a post mount system to minimize vibration from the camera's fans, or to accommodate an FTP system in lieu of a RAMM frame.)) with a ring clamp. The end of the tubes should come within approximately 1 mm from the upper (mirror) CUBE-IIIs. When the objectives are lowered into position for viewing a sample, adjust the bolts holding the tube support arms so the camera tube lenses are centered on the CUBE-IIIs. \\ 
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 **Goals:** **Goals:**
-The qualities of final alignment, for both SPIM optical paths, include all of the following: +The qualities of final alignment (for both SPIM optical paths), include all of the following: 
-  * epi spot centered on epi camera +  * beam/sheet plane coincident with imaging focus plane, near the center of the piezo and/or slice travel
-  * beam waist centered on imaging camera +
-  * beam/sheet plane coincident with imaging focus plane near the center of the piezo travel +
-  * beam perpendicular to imaging camera field of view+
   * sheet centered in imaging camera field of view   * sheet centered in imaging camera field of view
-  * sheets centered in bottom camera’s field of view (i.e. co-alignment of all three objectives)+  * beam perpendicular to imaging camera field of view 
 +  * beam waist centered on imaging camera 
 +  * for dual view, epi spot centered on epi camera 
 +  * when mounted on an inverted microscope, sheets centered in bottom camera’s field of view (i.e. co-alignment of all three objectives) 
 + 
 +For single-view systems there are more adjustments than required to achieve good alignment of the single light sheet plane and detection focal plane and does not have an epi view. This makes it easier to align, but also makes it possible to adjust things so that the illumination is far from the center of the illumination objective so this should be checked by eye.
  
 ==== Overview ====  ==== Overview ==== 

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